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ATCC Primary Cells

ATCC® Primary Cell Solutions® is a standardized cell culture system that includes high quality cells, media, supplements, reagents and protocols. ATCC Primary Cell Solutions provides researchers with superior quality from a trusted source.

• Cells are frozen at passage 1 through 3
• Tested for sterility and screened for viruses
• Guaranteed population doublings (10-15)
• > 70% viability
• > 80% positive marker expression
• < 10% negative marker expression

ATCC Primary Cell Solutions basal media and cell-specific growth kits are designed to support recovery and proliferation of ATCC Primary Cell Solutions primary cells. We supply human primary epithelial cells, fibroblasts, keratinocytes, melanocytes, endothelial cells, muscle cells, hematopoietic and immune cells, and mesenchymal stem cells derived from the major organ systems.  To learn more about ATCC primary cells

hTERT Immortalized Primary Cells

Primary cells closely represent the physiological state of a particular cell type in vivo, but they are susceptible to replicative senescence. Continuous cell lines, on the other hand, are not encumbered by replicative senescence, but, they often contain numerous genetic mutations, exhibit an unstable karyotype and have protein expression patterns that are not comparable with the cell type they are intended to represent. hTERT immortalized primary cells combine the physiological attributes of primary cell lines and the long culture life of continuous cell lines.  hTERT primary cells have been shown to maintain primary cell functionality at passage numbers greater than 35.

Solute Transporter Carrier Cells

Researchers need in vitro kidney transporter models that have normal human kidney origin, functioning transporters, clinical predictability, and consistent data output for drug interaction studies. Unfortunately, primary renal epithelial cells lose OAT1, OCT2, and OAT3 transporter expression with 24 hours in culture.

Using hTERT-immortalized primary renal proximal tubule epithelial cells, ATCC has been able to create kidney transporter cell models that stably overexpress either the OAT1, OCT2, or OAT3 gene, and maintain primary physiology with the proliferative capacity of a continuous cell line.

Application Notes

• Comprehensive Gene Expression Analysis and Neurotoxicity Testing of Human iPSC-derived Neural Progenitor Cells and Neurons
• Genetically modified renal proximal tubule epithelial cells

Culture Guides

• Animal Culture Guide
• hTert Culture Guide
• Primary Cell Culture Guide
• Stem Cell Culture Guide

ATCC Resources

• Toxicology Portfolio
• Custom Solutions 
• ATCC Primary Cells

Neural Progenitor Cells

Human iPSC-derived NPCs and neurons are an appealing resource for in vitro disease modeling, toxicity screening, and drug screening. ATCC normal and reporter-labeled NPCs have the potential to be differentiated into dopaminergic, GABAergic, glutamatergic, motor, and cholinergic neurons after treatment with ATCC Dopaminergic Differentiation Media. In addition, ATCC NPCs have been differentiated into astrocytes and oligodendrocytes. Live cell imaging and quantitative measurement of neuronal and glial differentiation is possible in the reporter-labeled NPCs with Nano Luciferase expression under the control of the GFAP or MAP2 gene promoter and GFP under control of the DCX promoter. Both NPCs and NPC-derived neurons have been validated for drug screening with several neurotoxicants by using a resazurin viability assay and high-content imaging assessment. ATCC NPCs are the ideal in vitro tool for identifying neurotoxicity in all of the major cell types of the nervous system.

hTERT Immortalized Neonatal Primary Melanocytes

hTERT-immortalized neonatal primary melanocytes were created by the retroviral transduction with the gene encoding the human telomerase catalytic subunit (hTERT) into a primary neonatal melanocyte.  Essential melanocyte characterization post-transduction was confirmed by immunofluorescence staining for the melanocyte marker TRP1. The hTERT-immortalized neonatal primary melanocytes cell line also retain the ability to produce melanin in culture at an excess of 35 PDLs, while maintaining  typical melanocyte morphology as observed through phase contrast microscopy.  The hTERT immortalized melanocytes in co-cultured with hTERT immortalized keratinocytes and dermal fibroblasts have been successfully used to create organotypic 3D skin models.